Our primary interest is the study of biochemical mechanisms of the obligate intracellular parasite, Toxoplasma gondii. This protozoan parasite infects an estimated one-third of North-Americans and is probably one of the most wide-spread parasite on earth. In general, the infections in healthy adults are usually asymptomatic. However, the infections in neonates and immuno-compromised individuals can be devastating. T.gondii is indeed the causative agent of congenital toxoplasmosis, the leading cause of neurological birth defects, and toxoplasmic encephalitis the most common cause of focal brain lesions in AIDS patients.
Fortunately T. gondii is an experimentally-accessible organism to which several molecular and genetic manipulation can be applied. The initial goal of the laboratory is to use antisense and ribozyme technology to disrupt the parasite nucleotide salvage pathway. The ultimate aims are to exploit these techniques to attack essential mechanisms of the parasite pathogenicity, such as invasion and differentiation.
- Selected Publications:
- Al-Anouti F, Tomavo S, Parmley S and Ananvoranich S (2004) The expression of lactate dehydrogenase is important for the cell cycle of Toxoplasma gondii Journal of Biological Chemistry 279: 52300 – 52311.
- Sheng J, Al-Anouti F and Ananvoranich S (2004) Engineered delta ribozymes can simultaneously knock down the expression of uracil phosphoribosyltransferase and hypoxanthine-xanthine-guanine phosphoribosyltransferase in Toxoplasma gondii. International Journal for Parasitololgy 34:253-263.
- Al-Anouti, F., Quach, T., and Ananvoranich S. (2003) Double-stranded RNA can mediate the suppression of uracil phosphoribosyltransferase expression in Toxoplasma gondii. Biochemical and Biophysical Research Communications 302: 316-323.
- Al-Anouti, F. and Ananvoranich S. (2002) Comparative analysis of antisense RNA, double-stranded RNA and delta ribozyme mediated gene regulation in Toxoplasma gondii. Antisense and Nucleic Acid Drug Development 4: 275-281.
- Ananvoranich, S., and Perreault J.P. (2000) The kinetics and magnesium requirements for the folding of antigenomic delta ribozymes. Biochemical and Biophysical Research Communications. 270: 600-607.
- Crater AK, Roscoe S, Fahim A, Ananvoranich S. Toxoplasma ubiquitin-like protease 1, a key enzyme in sumoylation and desumoylation pathways, is under the control of non-coding RNAs. Int J Parasitol. 2018, 48(11):867-880. doi: 10.1016/j.ijpara.2018.05.001.
- Fischer K, Roberts M, Roscoe S, Avci Y, Ananvoranich S. Toxoplasma gondii infection induces the formation of host's nuclear granules containing poly(A)-binding proteins. Can J Microbiol. 2018, 64(8):551-558. doi: 10.1139/cjm-2017-0755.
- Crater AK, Roscoe S, Roberts M, Ananvoranich S. Antisense technologies in the studying of Toxoplasma gondii. J Microbiol Methods. 2017, 138:93-99. doi: 10.1016/j.mimet.2015.12.013.
- Crater AK, Manni E, Ananvoranich S. Utilization of inherent miRNAs in functional analyses of Toxoplasma gondii genes. J Microbiol Methods. 2015, 108:92-102. doi: 10.1016/j.mimet.2014.11.014.
- Holmes M, Itaas V, Ananvoranich S. Sustained translational repression of lactate dehydrogenase 1 in Toxoplasma gondii bradyzoites is conferred by a small regulatory RNA hairpin. FEBS J. 2014, 281(22):5077-91. doi: 10.1111/febs.13048.
- Kadri D, Crater AK, Lee H, Solomon VR, Ananvoranich S. The potential of quinoline derivatives for the treatment of Toxoplasma gondii infection. Exp Parasitol. 2014, 145:135-44. doi: 10.1016/j.exppara.2014.08.008.
- Cherry AA, Ananvoranich S. Characterization of a homolog of DEAD-box RNA helicases in Toxoplasma gondii as a marker of cytoplasmic mRNP stress granules. Gene. 2014, 543(1):34-44. doi: 10.1016/j.gene.2014.04.011.
- Holmes M, Crater AK, Dhudshia B, Thadani AN, Ananvoranich S. Toxoplasma gondii: inhibitory activity and encystation effect of securinine and pyrrolidine derivatives on Toxoplasma growth. Exp Parasitol. 2011, 127(2):370-5. doi: 10.1016/j.exppara.2010.09.002.
- Holmes M, Liwak U, Pricop I, Wang X, Tomavo S, Ananvoranich S. Silencing of tachyzoite enolase 2 alters nuclear targeting of bradyzoite enolase 1 in Toxoplasma gondii. Microbes Infect. 2010, 12(1):19-27. doi: 10.1016/j.micinf.2009.09.010.
- Book Chapters:
- Ananvoranich, S., Fiola, K., Ouellet, J., Deschênes, P. and Perreault, J.P. Kinetic analysis of a bimolecular hepatitis delta ribozyme. Methods in Enzymology: Ribonucleases. Abelson, J. N. and Simon, M. I., eds Academic Press.
- Ananvoranich, S. and Perreault, J.P. Structure analysis of ribozymes. Encyclopedia of Analytical Chemistry: Instrumentation and Applications. Meyers, R. A. ed., John Wiley & Sons.